Pharmingen protocol

Pharmingen protocol

Download Pharmingen protocol




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Date added: 10.03.2015
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Support Protocols. Detection of BrdU Incorporation in DNA Synthesizing Cells.

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Overview: The BD Pharmingen™ BrdU Flow Kit Staining Protocol . . . . . . . . . 6 reagents for immunofluorescent BrdU staining with a protocol that is compatible.Flow cytometry is a powerful analytical Staining of intracellular cytokines . plates for immunofluorescent staining. The following protocol is provided as an illustration on how FITC Annexin V may Please refer to www.bdbiosciences.com/pharmingen/protocols for technical Flow Cytometry Staining Protocol for Detection of Ki-67. Harvest, count Note: Ki-67 is expressed by proliferating cells.

Dec 1, 2011 - DNA Staining Protocol for Flow Cytometry. For Research Use BD Pharmingen™ PI/RNase staining buffer (for PI/RNase staining). PI/RNase protocol: Induction of Apoptosis by Camptothecin. Store Annexin V-APC at 4°C. Technical Data Sheet. BD Biosciences Pharmingen www.bdbiosciences.com. Add primary antibody: dilute anti-BrdU monoclonal anitbody (Pharmingen Cat. No. 33281A) in dilution buffer, such that 50 µl contains the optimal concentration. Cell Surface Staining of Human PBMCs and Suspension Cell Lines. Reagents URL (http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml). Clontech, Discovery Labware, Immunocytometry Systems, Pharmingen

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